人颊黏膜微生物群落年龄相关性演替研究

[摘要] 目的 初步探讨健康人群颊黏膜菌群组成在不同年龄及牙列阶段的动态演替过程。方法 25例健康受试者根据年龄及牙列情况分为5组：乳牙列组、混合牙列组、青少年组、青年组及老年组；分别提取各样本的细菌总DNA进行聚合酶链式反应，采用Quantity One软件对扩增产物的变性梯度凝胶电泳（DGGE）指纹图谱进行生物信息学分

析。结果 1）颊黏膜菌群的组成具有个体差异性。2）乳牙列组、混合牙列组、青少年组、青年组及老年组DGGE指纹图谱的平均条带数分别为21.2±4.0、17.8±3.9、15.8±4.3、16.8±3.7、22.2±6.5，各组间的差异无统计学意义（P>

0.05），提示颊黏膜优势菌群的数量在各个年龄阶段保持相对稳定。3）5组的Shannon指数分别为1.73±0.2、1.43±0.1、1.05±0.2、1.45±0.2和1.63±0.3，各组间的差异有统计学意义（P=0.003）；提示颊黏膜微生物的多样性在儿童至青少年阶段呈下降趋势，在青年至老年阶段呈上升趋势。4）群落结构相似性聚类分析发现：同组内大部分样本聚类位置相近，群落结构表现出较高的相似性；不同组的大部分样本未聚类在一起，群落结构呈现出一定的差异性。结论 不同年龄组健康人群的颊黏膜微生物群落组成存在差异，青少年期可能是颊黏膜微生物多样性改变的重要转折点。

[关键词] 颊黏膜微生物群落； 微生物群落多样性； 年龄； 演替； 聚合酶链式反应-变性梯度凝胶电泳

[中图分类号] R 780.2 [文献标志码] A [doi] 10.7518/hxkq.2014.02.016

[Abstract] Objective This investigation aimed to examine how buccal mucosa microbiome succeeds in a healthy population with different ages and dentition stages. Methods Twenty-five subjects were recruited and subdivided into five groups： pri-mary dentition group， mixed dentition group， adolescent group， adult group， and elderly group. Individual mucosal microbiota was obtained by gently scraping both sides of the buccal mucosa with a cotton swab. Microbial diversity was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis （PCR-DGGE）. Results 1） The composition of buccal mucosa microbiota has great intra-individual divergence. 2） The average band numbers of the primary dentition group， mixed dentition group， adolescent group， adult group， and elderly group were 21.2±4.0， 17.8±3.9， 15.8±4.3， 16.8±3.7， and 22.2±6.5， respectively. No between-group differences was observed （P>0.05）， indicating that predominant strains in the oral cavity may be stable throughout an individual’s lifetime. 3） The Shannon indices of primary dentition group， mixed dentition group， adolescent group， adult group， and elderly group were 1.73±0.2， 1.43±0.1， 1.05±0.2， 1.45±0.2， and 1.63±0.3， respectively. A significant between-group difference was observed （P=0.003）， indicating that the microbial diversity of the buccal mucosa decreases from childhood through adolescence， but increases from adult through senescence. 4） The clustering analysis showed that most of the samples in the same group clustered together， indicating higher intra-group community structure similarity. Conclusion Composition of the buccal mucosa microbiota was different among age groups. Adolescence may be an essential turning point of microbial ecology succession throughout life.

推荐访问： 演替 黏膜 群落 相关性 微生物

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