南亚实蝇转录组及嗅觉相关基因的初步分析

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摘  要  南亞实蝇Bactrocera tau （Walker），是葫芦科、茄科蔬菜和多种热带、亚热带果树上的主要害虫，抗逆性强，繁殖速度快，防治难度大。为挖掘可用于生物防治的分子靶标和探讨副信息素类引诱剂对其作用的分子基础，本研究采用Illumina HiSeq2500 PE125 bp测序技术对其混合样进行转录组测序和生物信息学分析。经序列拼接获得36 109条Unigenes，进一步与七大公共数据库进行同源比对，注释了21 127条Unigenes，其中在Nr数据库中注释成功的Unigenes数目最多，20 948条。Nr数据库注释的南亚实蝇B. tau Unigenes与地中海实蝇Ceratitis capitata同源性最高，达59.80%;与模式昆虫黑腹果蝇Drosophila melanogaster的同源性次之，达14.86%。将Unigenes与GO数据库比对发现，14 029条Unigenes根据功能分为了3个大类57个亚类;而与KOG数据库比对结果为，13 479个Unigenes基因其功能属于25个类别;进一步KEGG数据库代谢分析表明，6442条Unigenes参与了5大类共152个代谢通路。基因注释进一步筛选鉴定获得了嗅觉相关基因528个，对其中气味结合蛋白基因氨基酸序列的分析表明，其大多为具有6个保守的半胱氨酸位点的典型气味结合蛋白，且与黑腹果蝇D. melanogaster、地中海实蝇C. Capicata、瓜实蝇B. cucuribitae、橘小实蝇B. dorsalis的气味结合蛋白基因具有很高的同源性。这为南亚实蝇对副信息素类引诱剂反应的相关功能基因的挖掘及从嗅觉上寻求科学的防治措施提供了基础数据。

关键词  南亚实蝇;转录组分析;高通量测序;基因注释;嗅觉基因中图分类号  S433      文献标识码  A

Preliminary Analysis of Transcriptome and Olfaction-related Genes in Bactrocera tau （Walker）

DU Yinggang^1，2， JI Qinge³， LAI Zhongxiong^2*

1. Facility Horticulture Laboratory of Universities in Shandong， Weifang University of Science and Technology， Shouguang， Shandong 262700， China; 2. Institute of Subtropical Pomology， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China; 3. College of Plant Protection， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China

Abstract  Bactrocera tau （Walker） is a major pest to Cucurbitaceous and Solanceous vegetables and many fruits of tropical and subtropical areas. Prevention and control of it is very difficult due to its strong resistance and rapid reproduction. To seek effective molecular targets for its control and the action mechanism of parapheromone， transcriptome of B. tau was sequenced and related bioinformatics analyzed using Illumina HiSeq2500 PE125 bp sequencing platform. The clean reads were then de novo assembled into 36 109 Unigenes. Through a similarity search against seven public databases， 21 127 unigenes were annotated. Most unigenes were annotated to Nr database with a success number of 20 948， and the unigenes of B. Tau had the highest homology to those of Ceratitis capitate in species distribution of unigenes in Nr database （59.80%）， followed by 14.86% with Drosophila melanogaster. According to GO database， 14 029 Unigenes were classified into 3 categories and 57 sub-types， while searching KOG database， 13 479 Unigenes were sorted in 25 functional groups. Metabolic analysis based on KEGG found 6442 Unigenes involved in 152 metabolite pathways in 5 classes. By further screening and identification， 528 olfaction-related genes of B. tau were obtained. Analysis of odorant binding protein genes of B. tau demonstrated that most of them were typical with 6 conserved cysteine sites and with high homology to those of D. melanogaster， C. capicata， B. cucuribita and B. dorsalis. These results will provid basic information for the research of functional genes related to parapheromone treatment on B. tau as well as the establishment of control measures based on olfactories.

推荐访问： 南亚 转录 嗅觉 基因 分析

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