樟树长链脂肪酰基CoA合成酶基因9克隆与表达分析

文章编号：1000-3142（2018）10-1335-11

Abstract：In this study，the cDNA encoding AtLACS9 homologous  gene was identified and cloned based on transcriptomes data of Cinnamomum camphora，sharing 75% sequence similarity to AtLACS9 and being registered to CcLACS9. Multiple sequences alignment showed CcLACS9 possessed three plant LACS-specific motifs and plastidical targeting signal in N-terminal. Using oleic acid as a substrate，CcLACS9 could activate free fatty acids into acyl-CoA thioesters in a yeast mutant deficient in LACS complementation test. The tissue-specific expression profile further revealed that CcLACS9 was predominantly expressed in developing seeds and flowers，but fewer in leaf and stem by real-time quantitative PCR analysis. There were 17.74-folds relative quantitative expression of CcLACS9 in kernels relative to roots. In an effort to better understand whether CcLACS9 involved seed oil biosynthesis in camphor kernel，the correlation between expression of CcLACS9 and seed oil content was surveyed. The seeds of thirty adult camphor trees were randomly sampled in November and thousand seed weight，seed oil content and the percentage of decanoic acid and lauric acid were tested and counted. According to seed oil content，the 30 individuals were subdivided into three groups and three representative plants were selected from each group to be subjected to association analysis between seed oil yield and CcLACS9 expression level in developing kernel. The results of three groups all showed that both the contents of seed oil and expression level of CcLACS9 continued to rise from June to August，and there were significant positive correlations between them. The peak of expression level of CcLACS9 in kernel was found in August. After September，the contents of seed oil tended to be stable，however，CcLACS9 keeped high expression level and had no correlation with contents of seed oil. In plant，the homoeologous genes of LACSs subfamily tend to carry the common function. These resluts implies that CcLACS9 possibly play an important role in seed oil accumulation in kernel of camphor tree.

推荐访问： 樟树 克隆 脂肪 基因 合成

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